Description
Mastitis is considered the most common and costly disease in the dairy industry. Detecting it early is easy and affordable with this cowside subclinical dipstick test.
- Mastitis is considered the most common and costly disease in the dairy industry. Detecting it early is easy and affordable with this cowside subclinical dipstick test.
- The Milk Mastitis LDH Test is designed to measure LDH (lactate dehydrogenase), an enzyme released into the milk when udder tissue is damaged and cells are compromised due to an infection.
- Studies show that measuring LDH activity in milk is an easy and cost-effective way to detect subclinical mastitis.
- Compared to SCC tests, testing for LDH is just as effective (if not more), is less expensive and provides earlier detection because LDH levels often increase earlier than SCC.
- While LDH is correlated to SCC, LDH is not as easily affected as by stress, nutrition, parity and stage of lactation, making it a more reliable indicator for determining an udder infection.
Use this reliable on-farm test to detect subclinical mastitis in individual quarters before it progresses and causes harm to both your cows and your profits. This test specifically measures and quantifies LDH, a common enzyme in milk that indicates tissue damage.
Simply dip this semiquantitative dipstick test into fresh milk, and then shake the dipstick and compare it to the provided color chart after just three minutes. To determine the level, use the easy-to-read color blocks at 0, 50, 100, 200, and 500 μmol/L LDH.
Instructions for use:
- Follow your normal pre-milking udder prep procedure and then strip each quarter 3-5 times.
- After the initial discard stripping, use the test as directed: Either strip milk directly onto the test pad or dip the test into a vial of milk. If testing a refrigerated sample, allow it to come to room temperature and shake the sample before testing. Quarter testing is recommended.
- Shake off excess milk from the strip and then wait three minutes before comparing it to the color chart.
Store at room temperature 36 ̊F – 77 ̊F (2 ̊C – 25 ̊C) and refrigerate whenever possible.
Note: Avoid using test strips that have discolored after extended storage. The reagent pad on unused test strips should be yellow.
Interpretation of results:
Test Results |
Infection Probability |
LDH Levels (U/L) |
– |
Low |
<100 |
+ |
Medium |
100-200 |
++ |
High |
200-500 |
+++ |
Very High |
>500 |
This test is intended solely for the estimation of LDH in fresh cow milk. The test is a screening tool and is not a laboratory reference method. It should not be used as a diagnostic test. Test results are not a diagnosis of disease. Consult a veterinarian before starting any treatment.
Frequently Asked Questions (FAQ)
What is this product?
The Milk Mastitis LDH Test offers a fast, affordable, and reliable way to detect subclinical mastitis in dairy cows. Mastitis is the most common and costly disease in the dairy industry, and early detection is crucial to prevent more significant losses. This test measures lactate dehydrogenase (LDH), an enzyme that is released when udder tissue is damaged due to infection. Using the Milk Mastitis LDH Test allows farmers to intervene early, saving both time and money by preventing further damage and avoiding milk loss.
When should I use this product?
The Milk Mastitis LDH Test is designed for early detection of subclinical mastitis, which does not show visible signs of infection. Early detection is essential, as it allows for intervention before the disease progresses and leads to significant costs. The test is ideal for:
- Monitoring problem cows: Quickly identify infected cows to prevent further spread.
- Checking cows at freshening and dry-off: Ensure the health of cows at these critical stages of lactation.
- Monitoring sick and treated cows: Track the progress of cows that are being treated for mastitis.
- Monthly herd screening: Regularly screen your herd to catch infections early.
- Screening cows at auction: Test cows before buying or selling to prevent the spread of mastitis.
- Before insemination: Ensure cows are infection-free before breeding.
Early Detection Advantage
Literature suggests that LDH levels peak before somatic cell count (SCC), offering the advantage of earlier detection. SCC based tests (such as CMT) lack the needed specificity because its affected by many other factors such as the number of lactations, stage of lactation, level of milk production, season, age and breed of cattle. Early detection is critical to maintaining both the health of your cows and the profitability of your farm.
How does this test compare to CMT?
The Milk Mastitis LDH Test compares favorably to other common mastitis detection methods, including SCC tests such as the California Mastitis Test (CMT):
- Early Detection: LDH levels often rise before SCC, which means detecting mastitis earlier. Early detection can save you money on medications, milk loss, and reduced milk premiums.It can also lead to better outcomes and herd health.
- Ease of Use: The Milk Mastitis LDH Test is cleaner and easier to use than CMT. Unlike CMT, which requires additional consumables like a paddle and solution, this test simply requires dipping a strip or stripping milk onto the test pad. No mess, no extra accessories—just dip, shake, and read.
- Clear Results: The Milk LDH Test provides clear, easily interpretable results, eliminating the subjectivity and guesswork associated with CMT. The color changes are straightforward, making it easy to understand the level of infection without any confusion.
Can this product be used for bulk tank testing?
LDH is stable in milk, so it is possible to measure LDH in bulk tanks, especially when the tank has just been filled. This allows farmers to test bulk tank milk shortly after collection. However, it’s important to note that in a smaller farm which takes days to fill its tank, multiple milkings might complicate the interpretation of the results. To minimize this risk, bulk tank samples should ideally come from a recently filled tank that contains only milk from the most recent milking.
How should I store this test?
Proper storage of the Milk Mastitis LDH Test kit is essential to ensure accuracy and longevity:
- Temperature range: Store the test kit between 2°C – 25°C (36°F – 77°F).
- Expiration: Use by the “Best If Used By” date printed on the vial.
- Avoid direct sunlight: Keep the kit and test strips away from direct sunlightÂ
- Avoid Humidity: Test strips can change color due to exposure to moisture. Since this test is used in the milking parlor, it is important that strips are removed with dry glovesÂ
What conditions should I run this test in?
To ensure the best results, run the test under the following conditions:
- Temperature range: Perform the test in an environment where the temperature is between 7°C and 35°C (45°F and 95°F).
- Shaded area: If testing outdoors, do so in a shaded area to protect the test from direct sunlight.
- Milk sample temperature: If the milk sample has been refrigerated, allow it to reach room temperature and mix the sample well before testing.
- Fresh samples: For the most accurate results, use the newest milk possible. However, the test is effective with milk that is up to 12 hours old and unrefrigerated, or up to 1 week old when refrigerated.
What are the requirements for the milk samples to get accurate results?
For best results, consider the following when using milk samples:
- Freshness: Use new raw milk, but milk up to 12 hours old (unrefrigerated) or 1 week old (refrigerated) can be tested effectively. The cow can be in any stage of lactation and does not need to be fresh to provide accurate results.
- Mixing: Always mix the milk sample thoroughly before testing to ensure consistency.
- Preservatives: Do not use milk with preservatives, as this can interfere with test results. Antibiotics do not affect the test.
- Temperature: Ensure the sample is at room temperature if it has been refrigerated.
Are there any precautions I should take when using or storing the strips?
To ensure accurate results with the Milk Mastitis LDH Test:
- Use within 15 minutes: Test strips should be used within 15 minutes of removal from the vial. After removing a strip, immediately reseal the vial to prevent exposure to moisture and light.
- Avoid contamination: Do not touch the reagent pad to prevent contamination. If dirt or water gets on the test strip, it may affect results.
- Proper storage: Always store test strips in the vial, tightly sealed, and away from direct light.
Handling: If the test strip is disturbed or dropped, it can still be used if no dirt or water is on it.
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