Pathogen transmission into zebrafish colonies is controlled through vigilant biosecurity practices. This study examined the practice is embryo surface disinfection, which often uses sodium hypochlorite. However, if sodium hypochlorite is used at an inappropriate pH, concentration, or exposure time, zebrafish embryos can experience significant mortality and morbidity. The study exposed zebrafish embryos to chlorine-containing agents to determine the impact on survival and defect issues. Bartovation’s chlorine test paper was utilized to provide an estimation of the amount of free chlorine available to verify the concentration.
